Mumps Research Today is a free monthly online journal that collates and summarizes the latest research about Mumps, including details on symptoms, vaccine, adult, causes.

Molecular identification of mumps virus genotypes from clinical samples: standardized method of analysis.

Palacios G, Jabado O, Cisterna D, de Ory F, Renwick N, Echevarria JE, Castellanos A, Mosquera M, Freire MC, Campos RH, Lipkin WI

Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, 722 W 168th Street, Fl. 18, New York, NY 10032, USA. gp2050@columbia.edu

A sensitive nested reverse transcription-PCR assay, targeting a short fragment of the gene encoding the small hydrophobic protein (SH gene), was developed to allow rapid characterization of mumps virus in clinical samples. The sensitivity and specificity of the assay were established using representative genotypes A, B, C, D, E, and F. Mumps virus RNA was characterized directly from cerebrospinal fluid (CSF) samples and in extracts of mumps virus isolates from patients with various clinical syndromes. Direct sequencing of products and subsequent phylogenetic analysis enabled genetic classification. A simple web-based system of sequence analysis was established. The study also allowed characterization of mumps virus strains from Argentina as part of a new subgenotype. This PCR assay for characterization of mumps infections coupled to a web-based analytical program provides a rapid method for identification of known and novel strains.

Published 7 April 2005 in J Clin Microbiol, 43(4): 1869-78.
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